Journal: Stem Cells and Development

Article Title: The Role of Cellular Proliferation in Adipogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells

doi: 10.1089/scd.2017.0071

Figure Lengend Snippet: Area measurements of stained oil droplets across different bFGF treatment groups demonstrated similar pattern as OilRedO quantification. (A) An exemplary image demonstrating how the ImagePro software identifies and outlines stained oil droplets and outlined areas was measured quantitatively. (B) The average value of area measurements from three independent experimental sets was calculated for each treatment group and graphed by comparing to the measurements of control group (n = 3). Color images available online at www.liebertpub.com/scd

Article Snippet: For quantification, cells were air-dried overnight in fume hood, extracted with pure isopropyl alcohol (Cat. No. A426P; Fisher Scientific; 100 μL/well for 24-well plate), transferred to 96-well plate, and OD was measured at 510 and 690 nm using Biotek Elx800 plate reader. bFGF treatment Frozen bFGF stock solution (Cat. No. 100-18B; PeproTech) at 2 μg/mL was freshly diluted into 2 ng/mL working solution in adipogenic differentiation media upon each use.

Techniques: Staining, Software

Journal: Chinese Medicine

Article Title: Huiyang Shengji decoction promotes wound healing in diabetic mice by activating the EGFR/PI3K/ATK pathway

doi: 10.1186/s13020-021-00497-0

Figure Lengend Snippet: HYSJD treatment accelerates wound closure in db/db diabetic mice. A Body weight of db/m and db/db mice. B Blood glucose levels ( n = 16 in each group). C Representative images of wound healing at days 3, 7, and 14 after wound surgery. D Wound closure analyzed using Image Pro ( n = 16, at day 7; n = 8, at day 14). E Hematoxylin and eosin staining of the wound healing tissues ondays 7 and 14 after wound surgery. Scale bar: 500 μm. Data were expressed as mean ± SD, and significance was expressed as ## P < 0.01 vs Ctrl and ** P < 0.01 vs Model. Ctrl, control; bFGF, basic fibroblast growth factor; HYSJD, Huiyang Shengji decoction

Article Snippet: A digital camera (Canon, Japan) was used to take images of the wounds. db/m mice that did not undergo the skin punch procedure were considered the control group (Ctrl group), n = 16; 48 db/db mice that underwent the skin punch procedure were randomly divided into 3 groups: (1) Model group (Model group), n = 16; (3) Positive control group, n = 16, topical treatment with basic fibroblast growth factor (bFGF) (3.6 μg/mL, PT 6358, PeproTech, USA), dissolved in normal saline (50 μL) to prepare a drug-containing gelatin sponge dressing (Nanjing Jinling Pharmaceutical Factory), and was applied to the wound externally; (4) HYSJD group, n = 16, HYSJD (19.93 g/kg) treatment.

Techniques: Staining, Control